Flexing the knee to isolate the single joint soleus from the biarticular gastrocnemius is a strategy forinvestigating individual plantarflexor's post activation potentiation (PAP). We investigated the implications of testingplantarflexor PAP at different knee angles and provided indirect quantification of the contribution of gastrocnemiuspotentiation to the overall plantarflexor enhancements post conditioning. Plantarflexor supramaximal twitches weremeasured in ten male power athletes before and after a maximal isometric plantarflexion (MVIC) at both flexed andextended knee angles. Mean torque and soleus (SOLRMS) and medial gastrocnemius (MGRMS) activity were measuredduring the MVIC. The mean torque and MGRMS of the MVIC were lower (by 33.9 and 42.4%, respectively) in the flexedcompared to the extended position, with no significant difference in SOLRMS. After the MVIC, twitch peak torque (PT)and the rate of torque development (RTR) potentiated significantly more (by 17.4 and 14.7% respectively) in theextended as compared to the flexed knee position, but only immediately (5 s) after the MVIC. No significant differenceswere found in the twitch rate of torque development (RTD) potentiation between positions. It was concluded that kneejoint configuration should be taken into consideration when comparing studies of plantarflexor PAP. Furthermore,results reflect a rather brief contribution of the gastrocnemius potentiation to the overall plantarflexor twitchenhancements.