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Mitochondrial efficiency in rat skeletal muscle: influence of respiration rate, substrate and muscle type.
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Research group for Mitokondriell funktion och metabolisk kontroll.
2006 (English)In: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 185, 229-236 p.Article in journal (Refereed) Published
Abstract [en]

Aim: To investigate the hypothesis that mitochondrial efficiency (i.e. P/O ratio) is higher in type I than in type II fibres during submaximal rates of respiration.

Methods: Mitochondria were isolated from rat soleus and extensor digitorum longus (EDL) muscles, representing type I and type II fibres, respectively. Mitochondrial efficiency (P/O ratio) was determined with pyruvate (Pyr) or palmitoyl-L-carnitine (PC) during submaximal (constant rate of ADP infusion) and maximal (Vmax, state 3) rates of respiration and fitted to monoexponential functions.

Results: There was no difference in Vmax between PC and Pyr in soleus but in EDL Vmax with PC was only 58% of that with Pyr. The activity of 3-hydroxyacyl-CoA dehydrogenase (HAD) was 3-fold higher in soleus than in EDL. P/O ratio at Vmax was 8-9% lower with PC (2.33±0.02 (soleus) and 2.30±0.02 (EDL)) than with Pyr (2.52±0.03 (soleus) and 2.54±0.03 (EDL)) but not different between the two muscles (P>0.05). P/O ratio was low at low rates of respiration and increased exponentially when the rate of respiration increased. The asymptotes of the curves were similar to P/O ratio at Vmax. P/O ratio at submaximal respirations was not different between soleus and EDL neither with Pyr nor with PC.

Conclusion: Mitochondrial efficiency, as determined in vitro, was not significantly different in the two fibre types neither at Vmax nor at submaximal rates of respiration. The low Vmax for PC oxidation in EDL may relate to low activity of β-oxidation.

Place, publisher, year, edition, pages
2006. Vol. 185, 229-236 p.
National Category
Dentistry
Identifiers
URN: urn:nbn:se:gih:diva-198OAI: oai:DiVA.org:gih-198DiVA: diva2:553
Note
The definitive version is available at www.blackwell-synergy.comAvailable from: 2007-05-08 Created: 2007-05-08 Last updated: 2011-05-06

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