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Metabolite levels in human skeletal muscle and adipose tissue studied with microdialysis at low perfusion flow.
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences.ORCID iD: 0000-0001-8161-5610
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences.ORCID iD: 0000-0002-8248-0348
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences.
1998 (English)In: The American journal of physiology, ISSN 0002-9513, Vol. 274, no 5 Pt 1, E936-45 p.Article in journal (Refereed) Published
Abstract [en]

To identify a perfusion flow at which the interstitial fluid completely equilibrates with the microdialysis perfusion fluid, a protocol with successively lower perfusion flows was used. A colloid was included in the perfusion fluid to make sampling possible at the lowest perfusion flows. At 0.16 microliter/min, the measured metabolites had reached a complete equilibration in both tissues, and the measured concentrations of glucose, glycerol, and urea were in good agreement with expected tissue-specific levels. The glucose concentration in adipose tissue (4.98 +/- 0.14 mM) was equal to that of plasma (5.07 +/- 0.07 mM), whereas the concentration in muscle (4.41 +/- 0.11 mM) was lower than in plasma and adipose tissue (P < 0.001). The concentration of lactate was higher (P < 0.001) in muscle (2.39 +/- 0.22 mM) than in adipose tissue (1.30 +/- 0.12 mM), whereas the glycerol concentration in adipose tissue (233 +/- 19.7 microM) was higher (P < 0.001) than in muscle (40.8 +/- 3.0 microM) and in plasma (68.7 +/- 3.97 microM). The concentration of urea was equal in the two tissues. Overall, the study indicates that microdialysis at a low perfusion flow may be a tool to continuously monitor tissue interstitial concentrations.

Place, publisher, year, edition, pages
1998. Vol. 274, no 5 Pt 1, E936-45 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:gih:diva-1066PubMedID: 9612253OAI: oai:DiVA.org:gih-1066DiVA: diva2:284561
Available from: 2010-01-07 Created: 2010-01-07 Last updated: 2017-03-01Bibliographically approved
In thesis
1. Microdialysis sampling from skeletal muscle and adipose tissue with special reference to the effects of insulin on tissue blood flow and glucose metabolism
Open this publication in new window or tab >>Microdialysis sampling from skeletal muscle and adipose tissue with special reference to the effects of insulin on tissue blood flow and glucose metabolism
1998 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Norstedts Tryckeri AB, 1998. 56 p.
Keyword
microdialysis, insulin, skeletal muscle, adipose tissue, glucose, amino acids
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:gih:diva-1082 (URN)91-628-3050-3 (ISBN)
Public defence
1998-06-12, Aulan, GIH, Lidingövägen 1, Stockholm, 09:00 (English)
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Supervisors
Available from: 2010-01-08 Created: 2010-01-08 Last updated: 2017-03-01Bibliographically approved

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