Eccentric exercises are commonly used as a treatment for various muscle and tendon injuries. During complex motions such as the forward lunge, however, it is not always clear which muscles may be contracting eccentrically and at what time. Because this exercise is used during rehabilitation, the purpose of this investigation was to determine what type of contractions take place during two different types of forward lunge and assess the implications for rehabilitation. Five experienced athletes performed five cycles for each of the walking and jumping forward lunges. Motion analysis was used to calculate the shortening or elongation of each muscle based on the change of position of their origin and insertion points during the lunge. Electromyography of the lateral hamstrings, rectus femoris and lateral gastrocnemius was combined with the muscle length change data to determine when isometric, concentric and eccentric activations occur during the lunge. Eccentric contractions in both the quadriceps and gastrocnemius were observed during the lunge. No hamstring eccentric contractions were found; however, the hamstrings showed isometric contractions during the first part of the stance phase.