An analysis of the take-off technique in transtibial amputee high jump was performed on two athletes (both using intact limb take-off) competing in the high jump finals of the 2004 Paralympic Games. Two digital video cameras were used to film the event with the data later digitized and reconstructed using standard 3D direct linear transformation (DLT) procedures. Some similarities with non-amputee high jump technique were noted in that centre of mass height was low at touch-down (TD), there was a similar magnitude of negative vertical velocity at TD, and most of the vertical velocity generated occurred in the first half of the take-off phase. However, both transtibial amputee athletes exhibited a slower horizontal approach velocity, a lower positive vertical take-off velocity, a more upright position at touch-down and a greater range of motion of the hip throughout the take-off phase compared to what is known about non-amputee high jump technique. These differences may be associated with constraints of taking off from the prosthetic limb on the previous step, resulting in having to adopt a different posture at touch-down compared to non-amputees. Understanding transtibial amputee high jump technique and the differences compared to what is known about non-amputee technique has implications for coaching and improving performance in prosthetic sport.