Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Increased autophagy signaling but not proteasome activity in human skeletal muscle after prolonged low-intensity exercise with negative energy balance
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology, Björn Ekblom's research group.ORCID iD: 0000-0003-3747-0148
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology.ORCID iD: 0000-0002-0642-4838
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology.ORCID iD: 0000-0002-3901-7833
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology, Research group for Mitokondriell funktion och metabolisk kontroll.ORCID iD: 0000-0002-7743-9295
Show others and affiliations
2017 (English)In: Physiological Reports, E-ISSN 2051-817X, Vol. 5, no 23, e13518Article in journal (Refereed) Published
Abstract [en]

Little is known about the molecular regulation of skeletal muscle protein turnover during exercise in field conditions where energy is intake inadequate. Here, 17 male and 7 female soldiers performed an 8 day long field based military operation. Vastus lateralis muscle biopsies, in which autophagy, the ubiquitin-proteasome system and the mTORC1 signaling pathway where studied, were collected before and after the operation. The 187 h long operation resulted in a 15% and 29% negative energy balance as well as a 4.1% and 4.6% loss of body mass in women and men respectively. After the operation protein levels of ULK1 as well as the phosphorylation of ULK1Ser317 and ULK1Ser555 had increased by 11%, 39% and 13%, respectively, and this was supported by a 17% increased phosphorylation of AMPKThr172 (P<0.05). The LC3b-I/II ratio was 3-fold higher after compared to before the operation (P<0.05), whereas protein levels of p62/SQSTM1 were unchanged. The β1, β2, and β5 activity of the proteasome and protein levels of MAFbx did not change, while levels of MuRF-1 were slightly reduced (6%, P<0.05). Protein levels and phosphorylation status of key components in the mTORC1 signaling pathway remained at basal levels after the operation. Muscle levels of glycogen decreased from 269±12 to 181±9 mmol ∙ kg dry muscle-1 after the exercise period (P<0.05). In conclusion, the 8 days of field based exercise resulted in induction of autophagy without any increase in proteasome activity or protein ubiquitination. Simultaneously, the regulation of protein synthesis through the mTORC1 signaling pathway was maintained.

Place, publisher, year, edition, pages
2017. Vol. 5, no 23, e13518
Keyword [en]
Energy deficit, ULK1, LC3b, proteolysis, mTORC1, military training
National Category
Cell and Molecular Biology
Research subject
Medicine/Technology
Identifiers
URN: urn:nbn:se:gih:diva-5096DOI: 10.14814/phy2.13518OAI: oai:DiVA.org:gih-5096DiVA: diva2:1159697
Available from: 2017-11-23 Created: 2017-11-23 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

fulltext(855 kB)2 downloads
File information
File name FULLTEXT01.pdfFile size 855 kBChecksum SHA-512
54cdffb1960c85c5f81e7838c0bbf7036a6f6518ec2cac6d8a10d8335c80a23607231d836d93a5a3457acdb783c35273f4d3ccab1442588b0194572a16a8bec6
Type fulltextMimetype application/pdf
sammanfattning(150 kB)0 downloads
File information
File name SUMMARY01.pdfFile size 150 kBChecksum SHA-512
01bc61be65ddf21d4f96bfbcf6687f49304632e18c31e3695ce66b7262c44d9c9248f746ad63371d44620d1b92be8973c4deb504e1625def849596c4a6a20c37
Type summaryMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Marcus, MobergMattsson, C MikaelEkblom-Bak, ElinFlockhart, MikaelPontén, MarjanSöderlund, KarinEkblom, Björn
By organisation
Björn Ekblom's research groupÅstrand Laboratory of Work PhysiologyResearch group for Mitokondriell funktion och metabolisk kontroll
In the same journal
Physiological Reports
Cell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 2 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 72 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf