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Regulation of UCP2 and UCP3 by muscle disuse and physical activity in tetraplegic subjects.
Karolinska institutet.ORCID iD: 0000-0001-9526-2967
1999 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 42, no 7, 826-30 p.Article in journal (Refereed) Published
Abstract [en]

AIMS/HYPOTHESIS: The regulation of uncoupling protein 2 and uncoupling protein 3 gene expression in skeletal muscle has recently been the focus of intense interest. Our aim was to determine expression of uncoupling protein 2 and 3 in skeletal muscle from tetraplegic subjects, a condition representing profound muscle inactivity. Thereafter we determined whether exercise training would modify expression of these genes in skeletal muscle.

METHODS: mRNA expression of uncoupling protein 2 and 3 was determined using quantitative reverse transcription-polymerase chain-reaction.

RESULTS: Expression of uncoupling protein 2 and 3 mRNA was increased in skeletal muscle from tetraplegic compared with able-bodied subjects (3.7-fold p < 0.01 and 4.1-fold, p < 0.05, respectively). A subgroup of four tetraplegic subjects underwent an 8-week exercise programme consisting of electrically-stimulated leg cycling (ESLC, 7 ESLC sessions/week). This training protocol leads to increases in whole body insulin-stimulated glucose uptake and expression of genes involved in glucose metabolism in skeletal muscle from tetraplegic subjects. After ESLC training, uncoupling protein 2 expression was reduced by 62% and was similar to that in able-bodied people. Similarly, ESLC training was associated with a reduction of uncoupling protein 3 expression in skeletal muscle from three of four tetraplegic subjects, however, post-exercise levels remained increased compared with able-bodied subjects.

CONCLUSION/INTERPRETATION: Tetraplegia is associated with increased mRNA expression of uncoupling protein 2 and 3 in skeletal muscle. Exercise training leads to normalisation of uncoupling protein 2 expression in tetraplegic subjects. Muscle disuse and physical activity appear to be powerful regulators of uncoupling protein 2 and 3 expression in human skeletal muscle.

Place, publisher, year, edition, pages
1999. Vol. 42, no 7, 826-30 p.
National Category
Neurology
Identifiers
URN: urn:nbn:se:gih:diva-5023DOI: 10.1007/s001250051233PubMedID: 10440124OAI: oai:DiVA.org:gih-5023DiVA: diva2:1143162
Available from: 2017-09-20 Created: 2017-09-20 Last updated: 2017-09-25Bibliographically approved

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