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Gene expression of the p85alpha regulatory subunit of phosphatidylinositol 3-kinase in skeletal muscle from type 2 diabetic subjects.
Karolinska institutet.ORCID iD: 0000-0001-9526-2967
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2002 (English)In: Pflügers Archiv: European Journal of Physiology, ISSN 0031-6768, E-ISSN 1432-2013, Vol. 445, no 1, 25-31 p.Article in journal (Refereed) Published
Abstract [en]

The gene of the p85alpha regulatory subunit of phosphatidylinositol (PI) 3-kinase gives rise to several splice variants. We hypothesized that the expression of p85alpha splice variants may be altered in skeletal muscle from subjects with type 2 diabetes mellitus. Skeletal muscle biopsies were obtained from nine type 2 diabetic and eight healthy men, matched for age, body mass index (BMI) and physical fitness. PI 3-kinase activity in skeletal muscle following in vitro insulin stimulation was reduced in subjects with type 2 diabetes. p85alpha mRNA was elevated fourfold in type 2 diabetic as compared to healthy control subjects ( P<0.05). p85alpha mRNA abundance was positively correlated with plasma insulin concentration ( P<0.01) and serum glucose concentration ( P<0.01). Despite this, protein levels of p85alpha, p55alpha, and the novel human p50alpha were not altered in type 2 diabetic subjects. Thus, although gene expression of full-length p85alpha is increased in skeletal muscle from type 2 diabetics, this is not reflected by increased protein levels. Therefore, defects in PI 3-kinase activity are likely due to impaired activation of the enzyme rather than changes in protein expression of the isoforms of the regulatory subunit.

Place, publisher, year, edition, pages
2002. Vol. 445, no 1, 25-31 p.
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:gih:diva-5021DOI: 10.1007/s00424-002-0907-9PubMedID: 12397383OAI: oai:DiVA.org:gih-5021DiVA: diva2:1143155
Available from: 2017-09-20 Created: 2017-09-20 Last updated: 2017-09-25Bibliographically approved

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