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Mitochondrial respiration is decreased in skeletal muscle of patients with type 2 diabetes.
University of Southern Denmark, Odense, Denmark.
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology, Research group for Mitokondriell funktion och metabolisk kontroll. University of Southern Denmark, Odense, Denmark.
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology. Karolinska institutet.
Odense University Hospital, Odense, Denmark.
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2007 (English)In: Diabetes, ISSN 0012-1797, E-ISSN 1939-327X, Vol. 56, no 6, 1592-9 p.Article in journal (Refereed) Published
Abstract [en]

We tested the hypothesis of a lower respiratory capacity per mitochondrion in skeletal muscle of type 2 diabetic patients compared with obese subjects. Muscle biopsies obtained from 10 obese type 2 diabetic and 8 obese nondiabetic male subjects were used for assessment of 3-hydroxy-Acyl-CoA-dehydrogenase (HAD) and citrate synthase activity, uncoupling protein (UCP)3 content, oxidative stress measured as 4-hydroxy-2-nonenal (HNE), fiber type distribution, and respiration in isolated mitochondria. Respiration was normalized to citrate synthase activity (mitochondrial content) in isolated mitochondria. Maximal ADP-stimulated respiration (state 3) with pyruvate plus malate and respiration through the electron transport chain (ETC) were reduced in type 2 diabetic patients, and the proportion of type 2X fibers were higher in type 2 diabetic patients compared with obese subjects (all P < 0.05). There were no differences in respiration with palmitoyl-l-carnitine plus malate, citrate synthase activity, HAD activity, UCP3 content, or oxidative stress measured as HNE between the groups. In the whole group, state 3 respiration with pyruvate plus malate and respiration through ETC were negatively associated with A1C, and the proportion of type 2X fibers correlated with markers of insulin resistance (P < 0.05). In conclusion, we provide evidence for a functional impairment in mitochondrial respiration and increased amount of type 2X fibers in muscle of type 2 diabetic patients. These alterations may contribute to the development of type 2 diabetes in humans with obesity.

Place, publisher, year, edition, pages
2007. Vol. 56, no 6, 1592-9 p.
National Category
Endocrinology and Diabetes
Research subject
Medicine/Technology
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URN: urn:nbn:se:gih:diva-5017DOI: 10.2337/db06-0981PubMedID: 17351150OAI: oai:DiVA.org:gih-5017DiVA: diva2:1143141
Available from: 2017-09-20 Created: 2017-09-20 Last updated: 2017-09-25Bibliographically approved

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