Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
INFLUENCE OF NUTRIENT INGESTION ON AMINO ACID TRANSPORTERS AND PROTEIN SYNTHESIS IN HUMAN SKELETAL MUSCLE AFTER SPRINT EXERCISE.
Karolinska Institutet .
Karolinska Institutet .
Huddinge University Hospital .
Karolinska Institutet.
Show others and affiliations
2017 (English)In: Journal of applied physiology, ISSN 8750-7587, E-ISSN 1522-1601, jap.00244.2017Article in journal (Refereed) Epub ahead of print
Abstract [en]

Nutrient ingestion is known to increase the exercise-induced stimulation of muscle protein synthesis following resistance exercise. Less is known about the effect of nutrients on muscle protein synthesis following sprint exercise. At two occasions separated by one month, twelve healthy subjects performed three 30-s sprints with 20-min rest between bouts. In randomized order, they consumed a drink with essential amino acids and maltodextrin (nutrient) or flavored water (placebo). Muscle biopsies were obtained 80 and 200 min after the last sprint and blood samples were taken repeatedly during the experiment. Fractional synthetic rate (FSR) was measured by continuous infusion of L-[(2)H5]-phenylalanine up to 200 min postexercise. The mRNA and protein expression of SNAT2 were both 1.4-fold higher (P < 0.05) after nutrient intake compared to placebo at 200 min postexercise. Phosphorylated Akt, mTOR and p70S6k was 1.7- to 3.6-fold higher (P<0.01) 80 min after the last sprint with nutrient ingestion as compared to placebo. In addition, FSR was higher (P<0.05) with nutrients when plasma phenylalanine (FSRplasma) was used as a precursor, but not when intracellular phenylalanine (FSRmuscle) was used. Significant correlations were also found between FSRplasma on the one hand and plasma leucine and serum insulin on the other hand in the nutrient condition. The results show that nutrient ingestion induces the expression of the amino acid transporter SNAT2, stimulates Akt/mTOR signaling and most likely the rate of muscle protein synthesis following sprint exercise.

Place, publisher, year, edition, pages
2017. jap.00244.2017
Keyword [en]
amino acids, high-intensity exercise, insulin, protein synthesis, skeletal muscle
National Category
Sport and Fitness Sciences Physiology
Research subject
Medicine/Technology
Identifiers
URN: urn:nbn:se:gih:diva-5011DOI: 10.1152/japplphysiol.00244.2017PubMedID: 28860165OAI: oai:DiVA.org:gih-5011DiVA: diva2:1142500
Available from: 2017-09-19 Created: 2017-09-19 Last updated: 2017-09-25Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Moberg, MarcusApró, WilliamBlomstrand, Eva
By organisation
Eva Blomstrand's research group
In the same journal
Journal of applied physiology
Sport and Fitness SciencesPhysiology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 12 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf