Gymnastik- och idrottshögskolan, GIH

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Acute hypoxia attenuates resistance exercise-induced ribosome signaling but does not impact satellite cell pool expansion in human skeletal muscle.
Swedish School of Sport and Health Sciences, GIH, Department of Physiology, Nutrition and Biomechanics.ORCID iD: 0000-0002-3500-2896
Swedish School of Sport and Health Sciences, GIH, Department of Physiology, Nutrition and Biomechanics. (Student)
Division of Pediatric Neurology, Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden..
Swedish School of Sport and Health Sciences, GIH, Department of Physiology, Nutrition and Biomechanics. Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Solna, Sweden..ORCID iD: 0000-0003-1942-2919
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2023 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 37, no 3, article id e22811Article in journal (Refereed) Published
Abstract [en]

Cumulative evidence supports the hypothesis that hypoxia acts as a regulator of muscle mass. However, the underlying molecular mechanisms remain incompletely understood, particularly in human muscle. Here we examined the effect of hypoxia on signaling pathways related to ribosome biogenesis and myogenic activity following an acute bout of resistance exercise. We also investigated whether hypoxia influenced the satellite cell response to resistance exercise. Employing a randomized, crossover design, eight men performed resistance exercise in normoxia (FiO2 21%) or normobaric hypoxia (FiO2 12%). Muscle biopsies were collected in a time-course manner (before, 0, 90, 180 min and 24 h after exercise) and were analyzed with respect to cell signaling, gene expression and satellite cell content using immunoblotting, RT-qPCR and immunofluorescence, respectively. In normoxia, resistance exercise increased the phosphorylation of RPS6, TIF-1A and UBF above resting levels. Hypoxia reduced the phosphorylation of these targets by ~37%, ~43% and ~ 67% throughout the recovery period, respectively (p < .05 vs. normoxia). Resistance exercise also increased 45 S pre-rRNA expression and mRNA expression of c-Myc, Pol I and TAF-1A above resting levels, but no differences were observed between conditions. Similarly, resistance exercise increased mRNA expression of myogenic regulatory factors throughout the recovery period and Pax7+ cells were elevated 24 h following exercise in mixed and type II muscle fibers, with no differences observed between normoxia and hypoxia. In conclusion, acute hypoxia attenuates ribosome signaling, but does not impact satellite cell pool expansion and myogenic gene expression following a bout of resistance exercise in human skeletal muscle.

Place, publisher, year, edition, pages
John Wiley & Sons, 2023. Vol. 37, no 3, article id e22811
Keywords [en]
Pax7, muscle fiber, myogenesis, resistance exercise, ribosome biogenesis
National Category
Physiology Sport and Fitness Sciences
Research subject
Medicine/Technology
Identifiers
URN: urn:nbn:se:gih:diva-7523DOI: 10.1096/fj.202202065RRISI: 000936598200001PubMedID: 36786723OAI: oai:DiVA.org:gih-7523DiVA, id: diva2:1741544
Funder
Swedish National Centre for Research in Sports, D2017- 0012, D2019- 0050, D2019- 0035Available from: 2023-03-06 Created: 2023-03-06 Last updated: 2023-03-23

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Horwath, OscarApro, WilliamMoberg, Marcus

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