Gymnastik- och idrottshögskolan, GIH

Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Nanowire-Imposed Geometrical Control in Studies of Actomyosin Motor Function
Linneuniversitetet.ORCID-id: 0000-0001-6878-3142
Vise andre og tillknytning
2015 (engelsk)Inngår i: IEEE Transactions on Nanobioscience, ISSN 1536-1241, E-ISSN 1558-2639, Vol. 14, nr 3, s. 289-297Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Recently, molecular motor gliding assays with actin and myosin from muscle have been realized on semiconductor nanowires coated with Al2O3. This opens for unique nanotechnological applications and novel fundamental studies of actomyosin motor function. Here, we provide a comparison of myosin-driven actin filament motility on Al2O3 to both nitrocellulose and trimethylchlorosilane derivatized surfaces. We also show that actomyosin motility on the less than 200 nm wide tips of arrays of Al2O3-coated nanowires can be used to control the number, and density, of myosin-actin attachment points. Results obtained using nanowire arrays with different inter-wire spacing are consistent with the idea that the actin filament sliding velocity is determined both by the total number and the average density of attached myosin heads along the actin filament. Further, the results are consistent with buckling of long myosin-free segments of the filaments as a factor underlying reduced velocity. On the other hand, the findings do not support a mechanistic role in decreasing velocity, of increased nearest neighbor distance between available myosin heads. Our results open up for more advanced studies that may use nanowire-based structures for fundamental investigations of molecular motors, including the possibility to create a nanowire-templated bottom-up assembly of 3D, muscle-like structures.

sted, utgiver, år, opplag, sider
2015. Vol. 14, nr 3, s. 289-297
Emneord [en]
Actin, aluminum oxide, in vitro motility assay, myosin, oxide-coated nanowire, sarcomere, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi
HSV kategori
Identifikatorer
URN: urn:nbn:se:gih:diva-6445DOI: 10.1109/TNB.2015.2412036OAI: oai:DiVA.org:gih-6445DiVA, id: diva2:1509847
Tilgjengelig fra: 2020-12-14 Laget: 2020-12-14 Sist oppdatert: 2020-12-21bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fullteksthttp://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-46290

Person

ten Siethoff, Lasse

Søk i DiVA

Av forfatter/redaktør
ten Siethoff, Lasse
I samme tidsskrift
IEEE Transactions on Nanobioscience

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 59 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf