Gymnastik- och idrottshögskolan, GIH

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Ultrafast molecular motor driven nanoseparation and biosensing
Linneuniversitetet.ORCID-id: 0000-0001-6878-3142
Vise andre og tillknytning
2013 (engelsk)Inngår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 48, s. 145-152Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Portable biosensor systems would benefit from reduced dependency on external power supplies as well as from further miniaturization and increased detection rate. Systems built around self-propelled biological molecular motors and cytoskeletal filaments hold significant promise in these regards as they are built from nanoscale components that enable nanoseparation independent of fluidic pumping. Previously reported microtubule-kinesin based devices are slow, however, compared to several existing biosensor systems. Here we demonstrate that this speed limitation can be overcome by using the faster actomyosin motor system. Moreover, due to lower flexural rigidity of the actin filaments, smaller features can be achieved compared to microtubule-based systems, enabling further miniaturization. Using a device designed through optimization by Monte Carlo simulations, we demonstrate extensive myosin driven enrichment of actin filaments on a detector area of less than 10 μm2, with a concentration half-time of approximately 40 s. We also show accumulation of model analyte (streptavidin at nanomolar concentration in nanoliter effective volume) detecting increased fluorescence intensity within seconds after initiation of motor-driven transportation from capture regions. We discuss further optimizations of the system and incorporation into a complete biosensing workflow.

sted, utgiver, år, opplag, sider
Elsevier , 2013. Vol. 48, s. 145-152
Emneord [en]
Actin filament; Diagnostics; Electron beam lithography; Heavy meromyosin; Nanoseparation; Monte-Carlo simulation, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi
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Identifikatorer
URN: urn:nbn:se:gih:diva-6446DOI: 10.1016/j.bios.2013.03.071OAI: oai:DiVA.org:gih-6446DiVA, id: diva2:1509838
Tilgjengelig fra: 2020-12-14 Laget: 2020-12-14 Sist oppdatert: 2020-12-21bibliografisk kontrollert

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ten Siethoff, Lasse

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