Gymnastik- och idrottshögskolan, GIH

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Nonlinear Cross-Bridge Elasticity and Post-Power-Stroke Events in Fast Skeletal Muscle Actomyosin
Linneuniversitetet.ORCID-id: 0000-0001-6878-3142
2013 (engelsk)Inngår i: Biophysical Journal, ISSN 0006-3495, E-ISSN 1542-0086, Vol. 105, nr 8, s. 1871-1881Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Generation-of force and movement by actomyosin cross-bridges is the molecular basis of muscle contraction, but generally accepted ideas about cross-bridge properties have recently been questioned. Of the utmost significance, evidence for nonlinear cross-bridge elasticity has been presented. We here investigate how this and other newly discovered or postulated phenomena would modify cross-bridge operation, with focus on post-power-stroke events. First, as an experimental basis, we present evidence for a hyperbolic [MgATP]-velocity relationship of heavy-meromyosin-propelled actin filaments in the in vitro motility assay using fast rabbit skeletal muscle myosin (28-29 degrees C). As the hyperbolic [MgATP]-velocity relationship was not consistent with interhead cooperativity, we developed a cross-bridge model with independent myosin heads and strain-dependent interstate transition rates. The model, implemented with inclusion of MgATP-independent detachment from the rigor state, as suggested by previous single-molecule mechanics experiments, accounts well for the [MgATP]-velocity relationship if nonlinear cross-bridge elasticity is assumed, but not if linear cross-bridge elasticity is assumed. In addition, a better fit is obtained with load-independent than with load-dependent MgATP-induced detachment rate. We discuss our results in relation to previous data showing a nonhyperbolic [MgATP1-velocity relationship when actin filaments are propelled by myosin subfragment 1 or full-length myosin. We also consider the implications of our results for characterization of the cross-bridge elasticity in the filament lattice of muscle.

sted, utgiver, år, opplag, sider
2013. Vol. 105, nr 8, s. 1871-1881
Emneord [en]
Biophysics, Biofysik
HSV kategori
Identifikatorer
URN: urn:nbn:se:gih:diva-6449DOI: 10.1016/j.bpj.2013.08.044OAI: oai:DiVA.org:gih-6449DiVA, id: diva2:1509831
Tilgjengelig fra: 2020-12-14 Laget: 2020-12-14 Sist oppdatert: 2020-12-21bibliografisk kontrollert

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ten Siethoff, Lasse

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