Gymnastik- och idrottshögskolan, GIH

Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Antibodies Covalently Immobilized on Actin Filaments for Fast Myosin Driven Analyte Transport
Linnéuniversitetet.ORCID-id: 0000-0001-6878-3142
Vise andre og tillknytning
2012 (engelsk)Inngår i: PLOS ONE, E-ISSN 1932-6203, Vol. 7, nr 10, artikkel-id e46298Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Biosensors would benefit from further miniaturization, increased detection rate and independence from external pumps and other bulky equipment. Whereas transportation systems built around molecular motors and cytoskeletal filaments hold significant promise in the latter regard, recent proof-of-principle devices based on the microtubule-kinesin motor system have not matched the speed of existing methods. An attractive solution to overcome this limitation would be the use of myosin driven propulsion of actin filaments which offers motility one order of magnitude faster than the kinesin-microtubule system. Here, we realized a necessary requirement for the use of the actomyosin system in biosensing devices, namely covalent attachment of antibodies to actin filaments using heterobifunctional cross-linkers. We also demonstrated consistent and rapid myosin II driven transport where velocity and the fraction of motile actin filaments was negligibly affected by the presence of antibody-antigen complexes at rather high density (>20 mu m(-1)). The results, however, also demonstrated that it was challenging to consistently achieve high density of functional antibodies along the actin filament, and optimization of the covalent coupling procedure to increase labeling density should be a major focus for future work. Despite the remaining challenges, the reported advances are important steps towards considerably faster nanoseparation than shown for previous molecular motor based devices, and enhanced miniaturization because of high bending flexibility of actin filaments.

sted, utgiver, år, opplag, sider
2012. Vol. 7, nr 10, artikkel-id e46298
HSV kategori
Identifikatorer
URN: urn:nbn:se:gih:diva-6443DOI: 10.1371/journal.pone.0046298OAI: oai:DiVA.org:gih-6443DiVA, id: diva2:1509828
Tilgjengelig fra: 2020-12-14 Laget: 2020-12-14 Sist oppdatert: 2021-06-14bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fullteksthttp://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-22698

Person

ten Siethoff, Lasse

Søk i DiVA

Av forfatter/redaktør
ten Siethoff, Lasse
I samme tidsskrift
PLOS ONE

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 45 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf