3132333435363735 of 37
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Isoproterenol enhances force production in mouse glycolytic and oxidative muscle via separate mechanisms.
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology.ORCID iD: 0000-0002-4853-6627
Swedish School of Sport and Health Sciences, GIH, Department of Sport and Health Sciences, Åstrand Laboratory of Work Physiology.
2019 (English)In: Pflügers Archiv: European Journal of Physiology, ISSN 0031-6768, E-ISSN 1432-2013, Vol. 471, no 10, p. 1305-1316Article in journal (Refereed) Published
Abstract [en]

Fight or flight is a biologic phenomenon that involves activation of β-adrenoceptors in skeletal muscle. However, how force generation is enhanced through adrenergic activation in different muscle types is not fully understood. We studied the effects of isoproterenol (ISO, β-receptor agonist) on force generation and energy metabolism in isolated mouse soleus (SOL, oxidative) and extensor digitorum longus (EDL, glycolytic) muscles. Muscles were stimulated with isometric tetanic contractions and analyzed for metabolites and phosphorylase activity. Under conditions of maximal force production, ISO enhanced force generation markedly more in SOL (22%) than in EDL (8%). Similarly, during a prolonged tetanic contraction (30 s for SOL and 10 s for EDL), ISO-enhanced the force × time integral more in SOL (25%) than in EDL (3%). ISO induced marked activation of phosphorylase in both muscles in the basal state, which was associated with glycogenolysis (less in SOL than in EDL), and in EDL only, a significant decrease (16%) in inorganic phosphate (Pi). ATP turnover during sustained contractions (1 s EDL, 5 s SOL) was not affected by ISO in EDL, but essentially doubled in SOL. Under conditions of maximal stimulation, ISO has a minor effect on force generation in EDL that is associated with a decrease in Pi, whereas ISO has a marked effect on force generation in SOL that is associated with an increase in ATP turnover. Thus, phosphorylase functions as a phosphate trap in ISO-mediated force enhancement in EDL and as a catalyzer of ATP supply in SOL.

Place, publisher, year, edition, pages
Springer, 2019. Vol. 471, no 10, p. 1305-1316
Keywords [en]
Fatigue, Force, Isoproterenol, Metabolites, Muscle, Phosphorylase
National Category
Physiology
Research subject
Medicine/Technology
Identifiers
URN: urn:nbn:se:gih:diva-5835DOI: 10.1007/s00424-019-02304-0PubMedID: 31451903OAI: oai:DiVA.org:gih-5835DiVA, id: diva2:1351709
Available from: 2019-09-16 Created: 2019-09-16 Last updated: 2019-11-06

Open Access in DiVA

fulltext(1534 kB)10 downloads
File information
File name FULLTEXT01.pdfFile size 1534 kBChecksum SHA-512
12fc51706290a4472dfcdad44656f8cfd25b9ca5b229b43397e45bba7f954303f8e141c2b823a7be28e9adf62fd916da4fc9f6efb64a30320aafdc2b74801b41
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Blackwood, Sarah JKatz, Abram

Search in DiVA

By author/editor
Blackwood, Sarah JKatz, Abram
By organisation
Åstrand Laboratory of Work Physiology
In the same journal
Pflügers Archiv: European Journal of Physiology
Physiology

Search outside of DiVA

GoogleGoogle Scholar
Total: 10 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 42 hits
3132333435363735 of 37
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf